{"id":"df2a93d3-0c36-4ccf-ae51-5a9621d392e6","secret":"read-only","organisms":["10090"],"elements":[{"id":"864b7d94-ef33-43c9-8ffe-594793b15fdf","secret":"read-only","position":{"x":247.0886075949367,"y":124.0506329113924},"name":"LRRTM4","description":"","type":"protein","completed":true,"association":{"combinedOrganismIndex":3,"dbName":"NCBI Gene","dbPrefix":"ncbigene","dbXrefs":[{"db":"MGI","id":"MGI:2389180"},{"db":"Ensembl","id":"ENSMUSG00000052581"}],"defaultOrganismIndex":3,"distance":0,"esScore":11.346347,"id":"243499","name":"Lrrtm4","nameDistance":0,"namespace":"ncbi","organism":"10090","organismIndex":0,"organismName":"Mus musculus","overallDistance":300000,"shortSynonyms":["7530419J18Rik","A230052N11","leucine-rich repeat transmembrane neuronal protein 4","leucine rich repeat transmembrane neuronal 4"],"synonyms":["7530419J18Rik","A230052N11","leucine-rich repeat transmembrane neuronal protein 4","leucine rich repeat transmembrane neuronal 4"],"type":"protein"}},{"id":"70158e6b-d105-4630-ace0-2064bfdd1ecf","secret":"read-only","position":{"x":449.6202531645569,"y":126.58227848101271},"name":"pikachurin","description":"","type":"protein","completed":true,"association":{"combinedOrganismIndex":0,"dbName":"NCBI Gene","dbPrefix":"ncbigene","dbXrefs":[{"db":"MGI","id":"MGI:2146149"},{"db":"Ensembl","id":"ENSMUSG00000042961"}],"defaultOrganismIndex":3,"distance":0,"esScore":11.243541,"id":"268780","name":"Egflam","nameDistance":1,"namespace":"ncbi","organism":"10090","organismIndex":0,"organismName":"Mus musculus","overallDistance":100,"shortSynonyms":["Egflam","5930412K08","AU040377","pikachurin","EGF-like, fibronectin type-III and laminin G-like domain-containing protein","nectican","EGF-like, fibronectin type III and laminin G domains"],"synonyms":["5930412K08","AU040377","pikachurin","EGF-like, fibronectin type-III and laminin G-like domain-containing protein","nectican","EGF-like, fibronectin type III and laminin G domains"],"type":"protein"}},{"id":"bc81a312-e8c3-4ec6-9e99-7ded3b3c46fd","secret":"read-only","position":{"x":32.22439585730707,"y":60.37245876486431},"name":"","description":"","type":"binding","completed":true,"association":"binding","novel":true,"entries":[{"group":null,"id":"864b7d94-ef33-43c9-8ffe-594793b15fdf"},{"group":"unsigned","id":"70158e6b-d105-4630-ace0-2064bfdd1ecf"}]}],"publicUrl":"/document/df2a93d3-0c36-4ccf-ae51-5a9621d392e6","privateUrl":"/document/df2a93d3-0c36-4ccf-ae51-5a9621d392e6","citation":{"title":"LRRTM4 is a member of the transsynaptic complex between rod photoreceptors and bipolar cells.","authors":{"abbreviation":"Melina A Agosto, Theodore G Wensel","contacts":[],"authorList":[{"name":"Melina A Agosto","ForeName":"Melina","LastName":"Agosto","email":null,"abbrevName":"Agosto MA","isCollectiveName":false,"orcid":"0000-0002-3647-1926"},{"name":"Theodore G Wensel","ForeName":"Theodore","LastName":"Wensel","email":null,"abbrevName":"Wensel TG","isCollectiveName":false,"orcid":null}]},"reference":"J Comp Neurol 529 2021","abstract":"Leucine rich repeat transmembrane (LRRTM) proteins are synaptic adhesion molecules with roles in synapse formation and signaling. LRRTM4 transcripts were previously shown to be enriched in rod bipolar cells (BCs), secondary neurons of the retina that form synapses with rod photoreceptors. Using two different antibodies, LRRTM4 was found to reside primarily at rod BC dendritic tips, where it colocalized with the transduction channel protein, TRPM1. LRRTM4 was not detected at dendritic tips of ON-cone BCs. Following somatic knockout of LRRTM4 in BCs by subretinal injection and electroporation of CRISPR/Cas9, LRRTM4 was abolished or reduced in the dendritic tips of transfected cells. Knockout cells had a normal complement of TRPM1 at their dendritic tips, while GPR179 accumulation was partially reduced. In experiments with heterologously expressed protein, the extracellular domain of LRRTM4 was found to engage in heparan-sulfate dependent binding with pikachurin. These results implicate LRRTM4 in the GPR179-pikachurin-dystroglycan transsynaptic complex at rod synapses.","pmid":"32390181","doi":"10.1002/cne.24944","pubTypes":[{"UI":"D016428","value":"Journal Article"},{"UI":"D052061","value":"Research Support, N.I.H., Extramural"},{"UI":"D013485","value":"Research Support, Non-U.S. Gov't"}],"ISODate":"2021-01-01T00:00:00.000Z","relations":[]},"text":"LRRTM4 binds with pikachurin.","createdDate":"2022-07-20T15:26:08.000Z","lastEditedDate":"2022-07-20T15:27:31.000Z","status":"public","verified":false,"authorProfiles":[{"ForeName":"Melina","LastName":"Agosto","abbrevName":"Agosto MA","email":null,"isCollectiveName":false,"name":"Melina A Agosto","orcid":"0000-0002-3647-1926"},{"ForeName":"Theodore","LastName":"Wensel","abbrevName":"Wensel TG","email":null,"isCollectiveName":false,"name":"Theodore G Wensel","orcid":null}]}